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Palier 1 • Identité NCBI 🦠 NCBI TAXID #28151 • SPECIES • Bacteria 🔎 NCBI Certificate #28151
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Serratia proteamaculans "Erwinia proteamaculans" (Paine and Stansfield 1919) Dye 1966

🌍 Psychrotrophic heat-resistant proteolysis, bitterness and rind liquefaction agent ; Agent psychrotrophe de protéolyse thermorésistante, d'amertume et de liquéfaction corticale

⚡ 3 active Index-Experts™ pole(s) (O(1) routing)  •  🧀 2 linked cheese matrix/matrices  •  🧊 24 3D structure(s) RCSB PDB / EBI SIFTS

🌳 Phylogenetic Lineage & Associated Cheese Matrices

Palier 2 • Fromager & Sommelier
Pseudomonadota (phylum) › Gammaproteobacteria (class) › Enterobacterales (order) › Yersiniaceae (family) › Serratia (genus)
🧀 Linked cheeses, dairy matrices and designations (2) :
🧀 Bries 🧀 Saint-Nectaire

🧫 Isolation Substrates, Biological Hosts & Cellar Ecology (NCBI Host)

Palier 3 • Praticien & Affineur
Laits réfrigérés de fromagerie, pâtes molles, pâtes pressées non cuites et pâtes filées (Mozzarella)
INDEX-EXPERT™ #01 • Tyrodexia™ Architecture Palier 5

Synonyms and historical names (NCBI)

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Nomenclature concordance (NCBI) Serratia proteamaculans ⟷ Erwinia proteamaculans • Pseudomonas proteamaculans • Xantomonas proteamaculans
Historical synonyms & basionyms Psychrotrophic heat-resistant proteolysis, bitterness and rind liquefaction agent ; Agent psychrotrophe de protéolyse thermorésistante, d'amertume et de liquéfaction corticale • Bacteria
Taxonomic authority & reference strains "Erwinia proteamaculans" (Paine and Stansfield 1919) Dye 1966
Official sources & reference book TaxID:28151 • Le grand dictionnaire du microbiome des fromages (https://www.tyrodexia.com/index.html#ouvrage)
Lineage & phylogenomic justification Pseudomonadota (phylum) › Gammaproteobacteria (class) › Enterobacterales (order) › Yersiniaceae (family) › Serratia (genus)
PubMed taxonomic references PMID:28624852 🔎 • DOI:10.1016/j.fm.2017.06.004 🔎
INDEX-EXPERT™ #06 • Tyrodexia™ Architecture Palier 3

Symptoms and manufacturing defects

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Vigilance category & defect diagnosis Agent psychrotrophe de protéolyse thermorésistante, d'amertume et de liquéfaction corticale • Amertume intense, texture pâteuse ou coulante sous croûte, perte de rendement en cuve et odeur putride-soufrée • Bries [en:bries], Saint-nectaire [en:saint-nectaire]
Spoilage metabolites & taxonomic lineage Sécrétion à basse température de la métalloprotéase dépendante du zinc serralysine (thermorésistante à la pasteurisation) et de lipases/décarboxylases
Documented substrates & historical synonyms Limitation du temps de stockage du lait cru réfrigéré (inférieur à 48 h à moins de 4 °C), NEP des tanks de collecte et acidification rapide en cuve • Psychrotrophie marquée (croissance active dès 2-4 °C), garde prolongée du lait cru, pH supérieur à 5.3, contamination hydrique ou végétale
Official sources & reference book TaxID:28151 • Le manuel clinique des pathologies laitières et fromagères (https://www.tyrodexia.com/index.html#collection)
Reference strain collections DSM 4543, ATCC 19323, NCTC 11554, CIP 103237
Operator safety & PubMed references PMID:28624852 🔎 • DOI:10.1016/j.fm.2017.06.004 🔎
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Pathogens and bioprotection

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Bacteriocins (NPClassifier) & health targets Flore de vigilance métagénomique (2 éch.) • Psychrotrophic heat-resistant proteolysis, bitterness and rind liquefaction agent ; Agent psychrotrophe de protéolyse thermorésistante, d'amertume et de liquéfaction corticale • Bacteria
Isolation substrates & linked cheese matrices Laits réfrigérés de fromagerie, pâtes molles, pâtes pressées non cuites et pâtes filées (Mozzarella) • Saint-nectaire [en:saint-nectaire], Bries [en:bries] • Pseudomonadota (phylum) › Gammaproteobacteria (class) › Enterobacterales (order) › Yersiniaceae (family) › Serratia (genus)
Physicochemical barriers (TDM, moisture, NaCl USDA) Saint-nectaire (EST 45.3 %, MNA 61.32 %, NaCl 1.22 g/100g) ; Bries (EST 51.6 %, MNA 66.94 %, NaCl 1.6 g/100g) • "Erwinia proteamaculans" (Paine and Stansfield 1919) Dye 1966 • Erwinia proteamaculans • Pseudomonas proteamaculans • Xantomonas proteamaculans
Official sources & reference book TaxID:28151 • Le manuel clinique des pathologies laitières et fromagères (https://www.tyrodexia.com/index.html#collection)
Prophylaxis & PubMed references PMID:28624852 🔎 • DOI:10.1016/j.fm.2017.06.004 🔎

⚠️ Cellar Clinical Vigilance & Generic Defects Monitoring (1)

Differential diagnosis of potential defects, biochemical mechanisms and cellar prophylaxis (Tier 3 — Practitioner):

⚠️ Psychrotrophic heat-resistant proteolysis, bitterness and rind liquefaction agent
ALTERATION_GUSTATIVE_AROME🔎 Proof
Amertume intense, texture pâteuse ou coulante sous croûte, perte de rendement en cuve et odeur putride-soufrée
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🏛️ Global Culture Collections (DSM / ATCC / CBS), NCBI Synonyms, Strains & FAO DAD-IS Breeds

Palier 4 • Institutionnel & Microbiologiste
NCBI Synonyms & Basionyms Erwinia proteamaculans • Pseudomonas proteamaculans • Xantomonas proteamaculans

🧬 RefSeq Genomics, 3D Structural Biology (EBI SIFTS), Metagenomics (cFMD/FMBN) & PubMed

Palier 5 • Expert Absolu
EBI SIFTS / RCSB PDB 3D Structures PDB #2VQX (24 crystallographic structures • CIF) — RCSB PDB 3D ↗
NCBI RefSeq Genomic Assembly RefSeq: GCA_949794035.1 • Taille: 5.5252 Mb • GC: 55.1439 % • Statut: Complete Genome • BioSample: SAMEA114445085 • BioProject: PRJEB65351
PubMed / DOI Primary Literature PMID:28624852 🔎 • DOI:10.1016/j.fm.2017.06.004 🔎
📊 Quantitative Metagenomic Detections (SegataLab/cFMD Cell 2024 & FoodMicrobionet) :
📊 SegataLab/cFMD (Cell 2024) : en:cheeses (n=24) 📊 SegataLab/cFMD (Cell 2024) : en:bries (n=2) 📊 SegataLab/cFMD (Cell 2024) : en:saint-nectaire (n=2)